Human trials,
not mouse studies.

Every ingredient in Dawn and Dusk, with the strongest human evidence behind it. Randomised controlled trials, meta-analyses and systematic reviews only, pulled from PubMed. Animal and test-tube work is excluded, which is most of what this industry quotes at you.

42Ingredients
2,682Human trials found
147Strongest shown
100%Have human evidence

Last scan 2026-09-08 · sorted by weight of evidence · every link goes to PubMed

L-Tryptophan

767
Dusk 250 mg

The raw material the body turns into serotonin, then melatonin. Trials measure sleep quality and mood.

Heavily studied · strongest papers

Vitamins B1, B3, B6, B12

262
Dawn 50 / 250 / 25 / 10 mg

Trials measure cognitive decline, nerve health, and fatigue during exercise.

Heavily studied · strongest papers

Ginkgo Biloba Extract

254
Dawn 100 mg

Trials measure cognition and anxiety. One review covers its interaction with blood thinners.

Heavily studied · strongest papers

Magnesium Glycinate

202
Dawn 500 mgDusk 1.1 g

Trials measure sleep quality, insomnia in older adults, muscle soreness, and subjective stress.

Heavily studied · strongest papers

Creatine Monohydrate

188
Dawn 2.5 g

Trials measure muscle strength, hypertrophy and memory. Kidney function was checked and found unaffected.

Heavily studied · strongest papers

N-Acetyl L-Glutamine

124
Dawn 375 mg

Trials measure gut barrier function, immune markers and recovery.

Heavily studied · strongest papers

CoQ10

101
Dawn 100 mg

Trials measure cardiovascular risk markers, cholesterol, inflammation and migraine frequency.

Heavily studied · strongest papers

Organic Beet Root

74
Dawn 200 mg

Trials measure endurance, aerobic capacity and repeated high-intensity effort.

Well studied · strongest papers

L-Citrulline

67
Dawn 4.5 g

Trials measure exercise performance, blood flow, and muscle strength in older adults.

Well studied · strongest papers

L-Theanine

65
Dawn 250 mgDusk 500 mg

Trials measure stress, sleep quality and cognition.

Well studied · strongest papers

Astragalus Extract

64
Dawn 250 mg

Trials measure immune and inflammatory markers. One found no improvement in muscle function.

Well studied · strongest papers

Taurine

57
Dawn 1.5 gDusk 750 mg

Trials measure endurance in heat, fatigue, and recovery after hard exercise.

Well studied · strongest papers

Inositol

49
Dawn 250 mgDusk 500 mg

Human trials concentrate on metabolic and reproductive health rather than sleep or calm.

Well studied · strongest papers

Lemon Balm Extract

45
Dusk 500 mg

Trials measure anxiety, mood and sleep quality.

Well studied · strongest papers

Rhodiola Extract

44
Dawn 150 mg

Trials measure stress, anxiety, cognition and exercise performance.

Well studied · strongest papers

Organic Gotu Kola

43
Dusk 250 mg

Human trials mostly cover skin ageing and circulation rather than cognition.

Well studied · strongest papers

Cordyceps Extract

37
Dawn 500 mg

Trials measure immune response. Safety was assessed in a randomised trial.

Well studied · strongest papers

NMN

34
Dawn 250 mg

Trials measure insulin sensitivity and physical performance in middle-aged and older adults, plus safety in healthy adults.

Well studied · strongest papers

Betaine Anhydrous

29
Dawn 600 mg

Trials measure cycling time-trial performance and anaerobic power. One found no hypertrophy benefit.

Well studied · strongest papers

Bacopa Extract

29
Dawn 250 mg

Trials measure memory, cognition and sleep quality.

Well studied · strongest papers

Huperzine A

21
Dawn 7.5 mg

Human trials are in Alzheimer's and dementia rather than healthy cognition.

Emerging · strongest papers

Blueberry Extract

18
Dawn 500 mg

Trials measure mood and dementia risk in midlife.

Emerging · strongest papers

Red + Black Maca Root

13
Dawn 250 mg each

Trials measure athletic performance, fatigue and sexual function. A review found testosterone claims unsupported.

Emerging · strongest papers

Organic ACV Powder

11
Dawn 250 mg

Trials measure blood sugar, cholesterol and body composition.

Emerging · strongest papers

Siberian Ginseng

10
Dawn 250 mg

Trials measure fatigue, burnout and cognition under stress.

Emerging · strongest papers

Uridine Monophosphate

9
Dawn 120 mg

Human trials are in early Alzheimer's, as part of a nutrient combination rather than alone.

Emerging · strongest papers

Alpha GPC

8
Dawn 300 mg

Trials measure cognitive performance in healthy adults, and isometric strength.

Emerging · strongest papers

Agmatine Sulfate

8
Dawn 500 mgDusk 500 mg

Human evidence is limited and centres on nerve pain rather than blood flow.

Emerging · strongest papers

Organic Lion's Mane

8
Dawn 1 gDusk 500 mg

Trials measure cognition, mood and anxiety.

Emerging · strongest papers

Nattokinase 4000 FU

7
Dawn 75 mgDusk 75 mg

Trials measure cardiovascular markers, cholesterol and circulation.

Emerging · strongest papers

Polygala Tenuifolia

6
Dawn 150 mg

Trials measure memory in healthy adults and cognition in older adults, plus safety.

Emerging · strongest papers

Magnolia Extract

6
Dusk 250 mg

Trials measure stress in healthy adults, and liver fat. The evidence base is small.

Emerging · strongest papers

Mag Acetyl L-Taurinate

5
Dusk 200 mg

Studied as taurine and magnesium together, measuring vascular function and wellbeing.

Thin evidence · strongest papers

Glycine

5
Dawn 1.5 gDusk 4.5 g

Trials measure glutathione production, oxidative stress, and muscle in critical illness.

Thin evidence · strongest papers

N-Acetyl L-Tyrosine

4
Dawn 500 mg

Trials measure endurance under mental fatigue and temperature regulation, plus safety in healthy men.

Thin evidence · strongest papers

Corydalis Extract

3
Dusk 500 mg

Human research is on pain relief rather than sleep. Two controlled trials exist.

Thin evidence · strongest papers

Phosphatidylserine

3
Dusk 150 mg

Trials measure cognitive performance, attention and vascular function.

Thin evidence · strongest papers

Sabroxy

2
Dawn 50 mg

Human evidence is very limited. Most published work is laboratory rather than clinical.

Thin evidence · strongest papers